| 质粒名称: | pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 N |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 189649 |
| 存储实验室: | Jin-Soo Kim |
|
载体骨架基本信息 |
|
| 载体名称: | pBAtC |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 12842bp |
| 载体类型: | Plant Expression, TALEN |
| 筛选标记: | Basta |
| 载体抗性: | Spectinomycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | TALE and DddAtox |
| 物种来源: | A. thaliana (mustard weed) |
|
文献引用方法 |
|
| 材料和方法部分: |
pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 N was a gift from Jin-Soo Kim (Addgene plasmid # 189649 ; http://n2t.net/addgene:189649 ; RRID:Addgene_189649)
|
| 参考文献部分: | Targeted A-to-G base editing of chloroplast DNA in plants. Mok YG, Hong S, Bae SJ, Cho SI, Kim JS. Nat Plants. 2022 Dec 1. doi: 10.1038/s41477-022-01279-8. 10.1038/s41477-022-01279-8 PubMed 36456803 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 N 12842 bp ds-DNA circular SYN 01-SEP-2022
DEFINITION rbc L 294 Left_G1397 N.
ACCESSION .
VERSION .
KEYWORDS pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 N
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12842)
TITLE sTALED for plants
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 12842)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 1, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..12842
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind complement(5..25)
/gene="mutant version of attB"
/label=attB4
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
CDS 1857..1922
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
misc_feature 5029..5053
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 6384..7013
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 7442..8515
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 8581..8775
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(9011..9033)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 9119..9259
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 9174..9193
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(9274..9291)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9445..10033)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9525..9544)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10279..11070)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
misc_feature 11591..11615
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
terminator 11708..11960
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(11980..12531)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/label=BlpR
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(12573..12752)
/label=NOS promoter
/note="nopaline synthase promoter"
ORIGIN
1 cgttcaactt ttctatacaa agttgaagat ctgactcaac ttttgattcg ctatttgcag
61 tgcacctgtg gcgttcatca catcttttgt gacactgttt gcactggtca ttgctattac
121 aaaggacctt cctgatgttg aaggagatcg aaagtaagta actgcacgca taaccatttt
181 ctttccgctc tttggctcaa tccatttgac agtcaaagac aatgtttaac cagctccgtt
241 tgatatattg tctttatgtg tttgttcaag catgtttagt taatcatgcc tttgattgat
301 cttgaatagg ttccaaatat caaccctggc aacaaaactt ggagtgagaa acattgcatt
361 cctcggttct ggacttctgc tagtaaatta tgtttcagcc atatcactag ctttctacat
421 gcctcaggtg aattcatcta tttccgtctt aactatttcg gttaattaaa gcacgaacac
481 cattactgca tgtagaagct tgataaacta tcgccaccaa tttatttttg ttgcgatatt
541 gttactttcc tcagtatgca gctttgaaaa gaccaaccct cttatccttt aacaatgaac
601 aggtttttag aggtagcttg atgattcctg cacatgtgat cttggcttca ggcttaattt
661 tccaggtaaa gcattatgag atactcttat atctcttaca tacttttgag ataatgcaca
721 agaacttcat aactatatgc tttagtttct gcatttgaca ctgccaaatt cattaatctc
781 taatatcttt gttgttgatc tttggtagac atgggtacta gaaaaagcaa actacaccaa
841 ggtaaaatac ttttgtacaa acataaactc gttatcacgg aacatcaatg gagtgtatat
901 ctaacggagt gtagaaacat ttgattattg caggaagcta tctcaggata ttatcggttt
961 atatggaatc tcttctacgc agagtatctg ttattcccct tcctctagct ttcaatttca
1021 tggtgaggat atgcagtttt ctttgtatat cattcttctt cttctttgta gcttggagtc
1081 aaaatcggtt ccttcatgta catacatcaa ggatatgtcc ttctgaattt ttatatcttg
1141 caataaaaat gcttgtacca attgaaacac cagctttttg agttctatga tcactgactt
1201 ggttctaacc aaaaaaaaaa aaatgtttaa tttacatatc taaaagtagg tttagggaaa
1261 cctaaacagt aaaatatttg tatattattc gaatttcact catcataaaa acttaaattg
1321 caccataaaa ttttgtttta ctattaatga tgtaatttgt gtaacttaag ataaaaataa
1381 tattccgtaa gttaaccggc taaaaccacg tataaaccag ggaacctgtt aaaccggttc
1441 tttactggat aaagaaatga aagcccatgt agacagctcc attagagccc aaaccctaaa
1501 tttctcatct atataaaagg agtgacatta gggtttttgt tcgtcctctt aaagcttctc
1561 gttttctctg ccgtctctct cattcgcgcg acgcaaacga tcttcaggtg atcttctttc
1621 tccaaatcct ctctcataac tctgatttcg tacttgtgta tttgagctca cgctctgttt
1681 ctctcaccac agccatggat tcacagctag tcttgtctct gaagctgaat ccaagcttca
1741 ctcctctttc tcctctcttc cctttcactc catgttcttc tttttcgccg tcgctccggt
1801 tttcttcttg ctactcccgc cgcctctatt ctccggttac cgtctacgcc gcgaaggact
1861 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
1921 agcccgggga tctacgcacg ctcggctaca gccagcagca acaggagaag atcaaaccga
1981 aggttcgttc gacagtggcg cagcaccacg aggcactggt cggccatggg tttacacacg
2041 cgcacatcgt tgcgctcagc caacacccgg cagcgttagg gaccgtcgct gtcaagtatc
2101 aggacatgat cgcagcgttg ccagaggcga cacacgaagc gatcgttggc gtcggcaaac
2161 agtggtccgg cgcacgcgct ctggaggcct tgctcacggt ggcgggagag ttgagaggtc
2221 caccgttaca gttggacaca ggccaacttc tcaagattgc aaaacgtggc ggcgtgaccg
2281 cagtggaggc agtgcatgca tggcgcaatg cactgacggg tgcccccctg aacctcacac
2341 cagatcaggt ggtggccatt gcaagcaata atggtggcaa gcaagccctc gaaaccgtcc
2401 aaaggctcct tcctgtcttg tgtcaggcac atggcctcac tccagaccag gtggtggcaa
2461 tcgcttctca tgatggagga aaacaagcac ttgagactgt tcagcggctt ttgcctgttc
2521 tttgccaaga ccatggactt accccagacc aggtcgttgc catcgcctca catgacggtg
2581 gaaagcaggc cttggaaaca gtgcaacgct tgctccccgt tctgtgccaa gctcatgggt
2641 tgactcctga tcaagtggtt gccattgcct ctaacaacgg cggaaaacag gctcttgaga
2701 ctgtccagag gctgctgccc gtgctctgtc aggatcatgg actcacacct gctcaagtgg
2761 ttgcaatcgc cagcaatatt ggcggtaaac aggccttgga aacagttcaa cgcttgcttc
2821 cagtcttgtg ccaagctcac gggctgaccc cagagcaggt cgtggctatc gcatctaaca
2881 atggtggtaa gcaagctctg gagacagtgc agagacttct cccagttctg tgccaggccc
2941 acggcctcac accagcacaa gtggtggcta tcgctagtaa cattggtggt aaacaagctc
3001 tggaaaccgt tcagcgcttg ctccctgtgc tgtgtcaaga tcacggattg acccctgccc
3061 aagtggtggc aatcgcttct aatgggggcg ggaagcaagc cctggagact gtgcagagac
3121 ttttgcccgt tctttgccag gatcatgggc tcaccccaga tcaggtcgtc gcaatcgctt
3181 ccaacatagg aggtaagcag gcactcgaaa cagttcaacg gctcctcccc gtgttgtgcc
3241 aagctcacgg tttgacccct gaacaagtcg tggctattgc tagtaacatc ggtggtaaac
3301 aggctcttga gacagttcaa agattgctgc cagtgttgtg tcaggctcac ggtttgactc
3361 cagctcaagt tgttgcaatc gccagtaacg gtggggggaa gcaggcactc gaaaccgtgc
3421 aacggcttct ccccgttctg tgccaggcac acggactgac accagcccaa gttgtcgcca
3481 tcgcttcaaa taacgggggt aaacaagctc tcgagacagt gcaacgcttg ttgcctgtgc
3541 tttgccaaga tcatgggttg actccagatc aggtggtggc cattgcaagc aataatggtg
3601 gcaagcaagc cctcgaaacc gtccaaaggc tccttcctgt cttgtgtcag gcacatggcc
3661 tcactccaga ccaggtggtg gcaatcgctt ctcatgatgg aggaaaacaa gcacttgaga
3721 ctgttcagcg gcttttgcct gttctttgcc aagaccatgg acttacccca gaccaggtcg
3781 ttgccatcgc ctcacatgac ggtggaaagc aggccttgga aacagtgcaa cgcttgctcc
3841 ccgttctgtg ccaagctcat ggattgaccc ctgaacaggt tgttgctatc gcatcaaatg
3901 gtggaggcaa gcaagctttg gaaaccgttc agcggctttt gccagtcctc tgtcaggcac
3961 acgggctcac accagatcag gtcgtcgcca tcgccagtaa tattggggga aagcaagcac
4021 ttgaaacagt gcaaagattg ttgcccgtgt tgtgccaagc acatggactc actcccgcac
4081 aagtggtcgc aattgccagc cacgacggcg ggaaacaagc cctcgaaact gtgcagaggc
4141 tcctccctgt cctctgtcaa gatcacggtc tgactccgga acaggtggtg gcgattgcaa
4201 gcaacggcgg cggcaaacag gctctagaga gcattgttgc ccagctctcc agacctgatc
4261 cggcgctagc cgcgttgaca aatgatcatc ttgttgctct tgcttgtctt ggcggacgac
4321 ctgccctgga tgctgtgaaa aaggggttgg ggggatccgg atcaggtagc tacgcacttg
4381 gtccttacca gattagcgca ccccaactcc ccgcctataa tggtcaaacc gtcgggacct
4441 tttactacgt aaacgatgct ggtgggctgg aatccaaagt attctcctca gggggcccta
4501 caccctaccc caactacgcc aatgctggtc atgtagaagg gcagtcagca ctgtttatgc
4561 gcgataatgg tataagcgag gggttggtct tccataacaa cccagagggt acttgtggct
4621 tctgtgtgaa tatgactgaa acccttctgc ccgaaaatgc caagatgact gtcgtcccac
4681 ctgaaggcta gggtacctga taatcgatat cccgcggcca tgctagagtc cgcaaaaatc
4741 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag
4801 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga
4861 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
4921 aaaccaaaat ccagtgacct gcaggcatgc gacgtccgtc gggcccaagc ttagcttgag
4981 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
5041 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
5101 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
5161 ggatcaaagt actttaaagt actttaaagt actttaaagt actttgatcc aacccctccg
5221 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
5281 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
5341 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
5401 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
5461 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
5521 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
5581 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
5641 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
5701 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
5761 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
5821 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
5881 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
5941 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
6001 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
6061 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
6121 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
6181 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
6241 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
6301 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
6361 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
6421 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
6481 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
6541 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
6601 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
6661 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
6721 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
6781 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
6841 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
6901 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
6961 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
7021 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
7081 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
7141 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
7201 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
7261 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
7321 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
7381 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
7441 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
7501 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
7561 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
7621 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
7681 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
7741 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
7801 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
7861 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
7921 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
7981 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
8041 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
8101 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
8161 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
8221 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
8281 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
8341 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
8401 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
8461 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
8521 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
8581 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
8641 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
8701 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
8761 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
8821 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
8881 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
8941 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
9001 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
9061 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
9121 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
9181 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
9241 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
9301 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
9361 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
9421 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
9481 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
9541 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
9601 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
9661 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
9721 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
9781 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
9841 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
9901 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
9961 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
10021 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
10081 aaactcacgt taagggattt tggtcatgca tgatatatct cccaatttgt gtagggctta
10141 ttatgcacgc ttaaaaataa taaaagcaga cttgacctga tagtttggct gtgagcaatt
10201 atgtgcttag tgcatctaac gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa
10261 cgaatttcta gctagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
10321 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
10381 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
10441 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
10501 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
10561 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
10621 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
10681 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
10741 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
10801 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
10861 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
10921 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
10981 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
11041 cgatacttcg gcgatcaccg cttcccccat gatgtttaac tttgttttag ggcgactgcc
11101 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
11161 ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat
11221 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
11281 tgagcgcata cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt
11341 tcgtgcccga attgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
11401 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
11461 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
11521 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
11581 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
11641 acacattgcg gacgttttta atgtactgaa ttaacgccga attgaattat cagcttgcat
11701 gccggtcgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
11761 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
11821 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
11881 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
11941 tgccaaatgt ttgaacgatc tgcttgactc taggggtcat cagatttcgg tgacgggcag
12001 gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca
12061 gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc
12121 gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc
12181 ctgtgcctcc agggacttca gaaggtgggt gtagagcgtg gagcccagtc ccgtccgctg
12241 gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt
12301 ccagggaccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga cgagccaggg
12361 atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt
12421 acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga ccgccggcat
12481 gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc
12541 cctcgatcga gttgagagtg aatatgagac tctaattgga taccgagggg aatttatgga
12601 acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact
12661 tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc
12721 gcggctcagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt
12781 cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcgataa
12841 tt
//
